These are the 2021 Opening Day rosters and Top 30 prospects for the Washington Nationals. MLB The Show 21 Opening Day rosters coming when the game releases later in April!
| Opening Day Roster – Washington Nationals |
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| Position | Last Name | First Name | Age | Level | Potential | OVR |
| SP | Scherzer | Max | 36 | MLB | ||
| SP | Strasburg | Stephen | 32 | MLB | ||
| SP | Corbin | Patrick | 31 | MLB | ||
| SP | Lester | Jon | 37 | MLB | ||
| SP | Ross | Joe | 27 | MLB | ||
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| Position | Last Name | First Name | Age | Level | Potential | OVR |
| RP | Rainey | Tanner | 28 | MLB | ||
| RP | Hudson | Daniel | 34 | MLB | ||
| RP | Suero | Wander | 29 | MLB | ||
| RP | Finnegan | Kyle | 29 | MLB | ||
| RP | Avilan | Luis | 31 | MLB | ||
| RP | Fedde | Erick | 28 | MLB | ||
| RP | Voth | Austin | 28 | MLB | ||
| RP | Harris | Will | 36 | A (INJ) | ||
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| Position | Last Name | First Name | Age | Level | Potential | OVR |
| CL | Hand | Brad | 31 | MLB | ||
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| Position | Last Name | First Name | Age | Level | Potential | OVR |
| C | Gomes | Yan | 33 | MLB | ||
| C | Avila | Alex | 34 | MLB | ||
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| Position | Last Name | First Name | Age | Level | Potential | OVR |
| 1B | Bell | Josh | 28 | MLB | ||
| 1B | Zimmerman | Ryan | 36 | MLB | ||
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| Position | Last Name | First Name | Age | Level | Potential | OVR |
| 2B | Harrison | Josh | 33 | MLB | ||
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| Position | Last Name | First Name | Age | Level | Potential | OVR |
| 3B | Castro | Starlin | 31 | MLB | ||
| 3B | Perez | Hernan | 30 | MLB | ||
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| Position | Last Name | First Name | Age | Level | Potential | OVR |
| SS | Turner | Trea | 27 | MLB | ||
| SS | Mercer | Jordy | 34 | MLB | ||
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| Position | Last Name | First Name | Age | Level | Potential | OVR |
| LF | Schwarber | Kyle | 28 | MLB | ||
| LF | Stevenson | Andrew | 26 | MLB | ||
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| Position | Last Name | First Name | Age | Level | Potential | OVR |
| CF | Robles | Victor | 23 | MLB | ||
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| Position | Last Name | First Name | Age | Level | Potential | OVR |
| RF | Soto | Juan | 22 | MLB | ||
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| Washington Nationals Top 30 Prospects via http://www.mlb.com | ||||||
| Rank | Last Name | First Name | Position | In Game? |
Potential | OVR |
| 1 | Cavalli | Cade | RHP | CAP | A | 60 |
| 2 | Rutledge | Jackson | RHP | Yes | ||
| 3 | Henry | Cole | RHP | CAP | B | 65 |
| 4 | Antuna | Yasel | SS | Yes | ||
| 5 | Lara | Andry | RHP | |||
| 6 | De La Rosa | Jeremy | RF | Yes | ||
| 7 | Cruz | Armando | SS | No | ||
| 8 | Cate | Tim | LHP | Yes | ||
| 9 | Denaburg | Mason | RHP | |||
| 10 | Cronin | Matt | LHP | Yes | ||
| 11 | Mendoza | Drew | 1B | Yes | ||
| 12 | Quintana | Roismar | ||||
| 13 | Infante | Sammy | ||||
| 14 | Marte | Daniel | ||||
| 15 | Pineda | Israel | C | Yes | ||
| 16 | Adon | Joan | RHP | No | ||
| 17 | Cluff | Jackson | ||||
| 18 | Romero | Seth | LHP | Yes | ||
| 19 | Barrera | Tres | C | Yes | ||
| 20 | Powell | Holden | RHP | No | ||
| 21 | Braymer | Ben | LHP | Yes | ||
| 22 | Fuentes | Steven | RHP | Yes | ||
| 23 | Dyson | Tyler | RHP | No | ||
| 24 | Lee | Evan | LHP | No | ||
| 25 | Irvin | Jake | RHP | No | ||
| 26 | Reetz | Jakson | C | Yes | ||
| 27 | Schaller | Reid | RHP | Yes | ||
| 28 | Pena | Viandel | ||||
| 29 | Sharp | Sterling | RHP | Yes | ||
| 30 | Sanchez | Bryan | RHP | No | ||
| N/A | Freeman | Cole | ||||
| N/A | Emiliani | Leandro | ||||
| N/A | Canning | Gage | ||||
| N/A | Hurtado | Jorge | ||||
